Oseltamivir resistance mutation N294S in human influenza A(H5N1) virus in Egypt

Earhart, Kenneth C.; Elsayed, Nasr M.; Saad, Magdi D.; Gubareva, Larisa V.; Nayel, Ahmed; Deyde, Varough M.; Abdelsattar, Ali; Abdelghani, Ahmad S.; Boynton, Bruce R.; Mansour, Moustafa M.; Essmat, Hala M.; Klimov, Alexander; Shuck-Lee, Deidra; Monteville, M.R.; Tjaden, Jeffrey A.

doi:10.1016/j.jiph.2009.04.004

« Previous Next »Journal of Infection and Public Health
Volume 2, Issue 2 , Pages 74-80, 2009

Oseltamivir resistance mutation N294S in human influenza A(H5N1) virus in Egypt☆

Kenneth C. Earhart
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
- I am an employee of the U.S. Government. This work was prepared as part of my official duties. Title 17 U.S.C. §105 provides that ‘Copyright protection under this title is not available for any work of the United States Government’ Title 17 U.S.C. §101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person's official duties.
- Corresponding author. United States Naval Medical Research Unit No. 3, PSC 452 Box 5000, MDS - Code 303, FPO AE 09835-0007, Egypt. Tel.: +20 223421381; fax: +20 223421382.
Nasr M. Elsayed
- Ministry of Health, Cairo, Egypt
Magdi D. Saad
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
Larisa V. Gubareva
- U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA
Ahmed Nayel
- Ministry of Health, Cairo, Egypt
Varough M. Deyde
- U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA
Ali Abdelsattar
- Ministry of Health, Cairo, Egypt
Ahmad S. Abdelghani
- Ministry of Health, Cairo, Egypt
Bruce R. Boynton
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
Moustafa M. Mansour
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
Hala M. Essmat
- Ministry of Health, Cairo, Egypt
Alexander Klimov
- U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA
Deidra Shuck-Lee
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
M.R. Monteville
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
Jeffrey A. Tjaden
- U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Received 23 February 2009; received in revised form 30 March 2009; accepted 1 April 2009.

Summary

In December 2006, three human specimens were received that were suspected positive for influenza A(H5N1). The specimens were tested using real time PCR. And the presence of A(H5N1) virus was confirmed in 2 patients (16F and 26M), The NA sequence from A(H5N1) positive specimens collected before and after antiviral therapy revealed a mutation (N294S) (N295S according to N1 numbering), previously associated with resistance to oseltamivir. When tested with NA inhibition assays, the two N294S viruses from Egypt exhibited from 57 to 138-fold reduction in susceptibility to oseltamivir, depending on the assay. To our knowledge, this is the first time oseltamivir resistance has been detected in A(H5N1) infecting a human prior to treatment.

Keywords: Neuraminidase inhibitors, Oseltamivir resistance, Influenza A virus, A(H5N1), Egypt

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☆ Disclaimer: The views and opinions expressed herein do not necessarily represent those of the U.S. Army or U.S. Navy or the Department of Defense.

PII: S1876-0341(09)00031-8

doi:10.1016/j.jiph.2009.04.004

Published by Elsevier Inc.

« Previous Next »Journal of Infection and Public Health
Volume 2, Issue 2 , Pages 74-80, 2009

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