Journal of Infection and Public Health
Volume 2, Issue 4 , Pages 184-188 , 2009

A combined molecular typing approach does not discriminate Legionella pneumophila serogroup 1 strains of a predominant sequence-based type in Palermo, Italy

  • Celestino Bonura

      Affiliations

    • Section of Microbiology, Department of Sciences for Health Promotion “G. D’Alessandro”, University, Palermo, Italy
    • ,
  • Caterina Mammina

      Affiliations

    • Section of Hygiene, Department of Sciences for Health Promotion “G. D’Alessandro”, University, Via del Vespro 133, I-90127 Palermo, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 0916553623; fax: +39 0916553641.
    • ,
  • Antonietta Vella

      Affiliations

    • Section of Microbiology, Department of Sciences for Health Promotion “G. D’Alessandro”, University, Palermo, Italy
    • ,
  • Santina Belfiore

      Affiliations

    • Public Health Laboratory, Azienda Sanitaria Locale 6, Palermo, Italy
    • ,
  • Alfredo Chiarini

      Affiliations

    • Section of Microbiology, Department of Sciences for Health Promotion “G. D’Alessandro”, University, Palermo, Italy
    • ,
  • Anna Giammanco

      Affiliations

    • Section of Microbiology, Department of Sciences for Health Promotion “G. D’Alessandro”, University, Palermo, Italy

Received 7 July 2009 ,Revised 7 September 2009 ,Accepted 10 September 2009.

References 

  1. Schuetz AN, Hughes RL, Howard RM, Williams TC, Nolte FS, Jackson D, et al. Pseudo-outbreak of Legionella pneumophila serogroup 8 infection associated with a contaminated ice machine in a bronchoscopy suite. Infect Control Hosp Epidemiol. 2009;30:461–466
  2. Casini B, Valentini P, Baggiani A, Torracca F, Frateschi S, Nelli LC, et al. Molecular epidemiology of Legionella pneumophila serogroup 1 isolates following long-term chlorine dioxide treatment in a university hospital water system. J Hosp Infect. 2008;69:141–147
  3. Ginevra C, Duclos A, Vanhems P, Campèse C, Forey F, Lina G, et al. Host-related risk factors and clinical features of community-acquired legionnaires disease due to the Paris and Lorraine endemic strains, 1998–2007, France. Clin Infect Dis. 2009;49:184–191
  4. Gaia V, Fry NK, Afshar B, Lück PC, Meugnier H, Etienne J, et al. Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J Clin Microbiol. 2005;43:2047–2052
  5. Ratzow S, Gaia V, Helbig JH, Fry NK, Lück PC. Addition of neuA, the gene encoding N-acylneuraminate cytidyl-transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. J Clin Microbiol. 2007;45:1965–1968
  6. Huang B, Heron BA, Gray BR, Eglezos S, Bates JR, Savill J. A predominant and virulent Legionella pneumophila serogroup 1 strain detected in isolates from patients and water in Queensland, Australia, by an amplified fragment length polymorphism protocol and virulence gene-based PCR assay. J Clin Microbiol. 2004;42:4164–4168
  7. Amemura-Maekawa J, Kura F, Chang B, Watanabe H. Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster. Microbiol Immunol. 2005;49:1027–1033
  8. Chiarini A, Bonura C, Ferraro D, Barbaro R, Calà C, Distefano S, et al. Genotyping of Legionella pneumophila serogroup 1 strains isolated in Northern Sicily, Italy. New Microbiol. 2008;3:217–228
  9. Borchardt J, Helbig JH, Lück PC. Occurrence and distribution of sequence types among Legionella pneumophila strains isolated from patients in Germany: common features and differences to other regions of the world. Eur J Clin Microbiol Infect Dis. 2008;27:29–36
  10. Fry NK, Afshar B, Bellamy W, Gaia V, Lück CP, Etienne J, et al. Update on the consensus sequence-based epidemiological typing scheme for Legionella pneumophila. The 20th annual meeting European Working Group for Legionella Infection, Rome, Italy. ISTISAN Congressi 05/C2 [Abstract book] p. 32; 2005.
  11. Wong S, Pabbaraju K, Burk VF, Broukhanski GC, Fox J, Louie T, et al. Use of sequence-based typing for investigation of a case of nosocomial legionellosis. J Med Microbiol. 2006;55:1707–1710
  12. Schoonmaker D, Heimberger T, Birkhead G. Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak. J Clin Microbiol. 1992;30:1491–1498
  13. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33:2233–2239
  14. Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: an application of Sympson's index od diversity. J Clin Microbiol. 1988;26:2465–2466
  15. Söderberg MA, Dao J, Starkenburg SR, Cianciotto NP. Importance of type II secretion for survival of Legionella pneumophila in tap water and in amoebae at low temperatures. Appl Environ Microbiol. 2008;74:5583–5588
  16. Oberdorfer K, Mossigbrodt G, Wendt C. Genetic diversity of Legionella pneumophila in hospital water systems. Int J Hyg Environ Health. 2008;211:172–178

PII: S1876-0341(09)00064-1

doi: 10.1016/j.jiph.2009.09.003

Journal of Infection and Public Health
Volume 2, Issue 4 , Pages 184-188 , 2009

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